Differential chemokine responses among NKT cell subsets.

(A) Chemotactic responses of CD4, CD8, and DN NKT cell subsets. Cell migration is shown as net migration after subtraction of background migration. (B) Frequency of IL-2–, IL-4–, and IFN-γ–producing NKT cells in chemotaxed cells after migration to TARC or LARC. Chemokines were used at the indicated concentration (A) or 250 ng/mL (TARC) and 1500 ng/mL (LARC) for panel B. Chemotaxed NKT cells were phenotyped by surface and/or intracellular cytokine staining followed by counting. Relative frequencies of cytokine producers were calculated by dividing numbers of cytokine-producing NKT cells after chemotaxis by those NKT cells in input (input = 1, marked by horizontal lines [B]). *Significant differences (P < .05) between CD4 and CD8 or DN NKT subsets (n = 4, [A]) or the 2 groups (n = 6 [B]).