Flow sorting of CD34⁺CD19⁻cells.

(A) Presort analysis and sort gates for CD34⁺CD19⁻ and CD34⁺CD19⁺ cells in the leukemic bone marrow of patient no. 1523/00. (B) Reanalysis of flow-sorted CD34⁺CD19⁻ cells from patient no. 1523/00 showing a purity of 94% and 4% contaminating CD19⁺ cells (sorter errors). The fluorescence intensity for CD34–fluorescein isothiocyanate was always lower on reanalysis, as expected from the initial sort gates.