RT-PCR analysis of flow-sorted CD34⁺CD19⁻cells.

(A) Representative agarose gel electrophoresis of the nested PCR forTEL/AML1 and GAPDH (single-step PCR) from 1000 sorted CD34⁺CD19⁻ (+−), CD34⁺CD19⁺ (++) cells, and REH control cells. When high numbers of TEL/AML1 transcripts were present in the samples (eg, ++ cells in patient nos. 1007/99 and 1523/00; 100% and 10% REH controls), the slightly longer product from the first PCR reaction was coamplified (upper band). (B) Semiquantitative real-time analysis of the second PCR reaction for TEL/AML1. Arrows indicate the crossing points of the respective controls (broken lines), and numbers identify the different patients (solid lines; 1, no. 807/00; 2, no. 1007/99; 3, no. 1523/00; 4, no. 589/97).