NF-Y expression in BM cells treated with MCSF plus IL-6.

(A) JunB expression in BM following stimulation with MCSF (10%) plus IL-6 (100 ng/mL), for indicated times. Preparation of RNA and Northern blot analysis was performed as mentioned earlier. (B) Nuclear extracts (5 μg) from untreated and treated (MCSF plus IL-6 for 3 days) BM were incubated with JunBWT probe (10 000 cpm). EMSA was performed as indicated in “Materials and methods.” (C) EMSA analysis was done as in B, except extracts from treated cells were incubated with antibody, as indicated for each sample, prior to addition of probe, as in Figure2. (D) NF-Y protein expression in BM. Western blot analysis and probing with antibodies were performed as previously described.