KGF treatment does not affect the severity of splenic GVHD.

Acute GVHD was induced in unirradiated B6D2F₁ mice as in Figure 1 and recipients were analyzed on day 13 after transplantation. (A) Spleen weights (mg) and (B) cellularities (cells × 10⁶) in transplant recipients treated with KGF (▧) or HBSS (■). HBSS-treated B6D2F₁ mice that received syngeneic transplants served as non-GVHD controls (▪). (C) Infiltration of donor T cells into the spleen (in percent, left panel; total cell number × 10⁶ right panel) was determined. Donor-derived T cells were distinguished from host cells (CD45.2⁺) by their expression of CD45.1. (D) The surface expression of CD4 and CD8 on splenocytes was analyzed by flow cytometry and quantified (% of total splenocytes). The graphs represent pooled data from 3 independent experiments, with 10 mice analyzed for each group. Analysis by ANOVA; *P < .05.