KGF modulates gene expression in thymi from mice that received transplants.
Acute GVHD was induced in unirradiated B6D2F1 mice, as described in Figure 1. Recipients of allogeneic T cells were treated with either KGF (▧) or HBSS (■). HBSS-treated B6D2F1mice that received syngeneic transplants served as non-GVHD controls (▪). RNA isolated from whole thymic tissue was analyzed 13 days after transplantation by quantitative RT-PCR for transcripts specific for IL-7, TECK, and Aire (A) and for Mip-1α, Mip-1β, and granzyme B (B), respectively. TEC-derived transcripts (TECK, IL-7, Aire) were normalized for EVA expression whereas hematopoietic cell–specific transcripts (granzyme B, Mip-1α, and Mip-1β) were normalized for GAPDH expression. Each graph represents pooled data from one experiment, with 3 mice analyzed for each group. Analysis by ANOVA; *P < .05.
