KGF reduces CD80 expression on thymic stromal cells in recipients with GVHD.
Acute GVHD was induced in KGF-treated or HBSS-treated B6D2F1 mice, as described in Figure 1. (A) At 13 days after transplantation, frozen thymic sections (6 μm) from the 3 transplant groups were stained with anti–CD80 moAb. Slides were then counterstained with hemalaun. Adjacent sections (not shown) were stained for cytokeratins in order to verify the presence of TECs. Original magnification × 200. (B) RNA isolated from whole thymic tissue was analyzed 13 days after transplantation by end-point RT-PCR for transcripts specific for CD80. Transcripts were normalized for EVA expression. The graph represents data from 3 mice analyzed for each group. Analysis by ANOVA; *P < .05.
