Inhibitory effect of the anti-GPIa monoclonal antibody, Gi9, and free rGPIa/IIa on the interaction of rGPIa/IIa-liposomes with the collagen surface under flow conditions.
Control: images were obtained after 3 minutes of perfusion at a shear rate of 600 s−1. Liposomes have an exofacial rGPIa/IIa density of 2.22 × 103 molecules per particle. Anti-GPIa: experimental conditions were the same as control except that rGPIa/IIa-liposomes were incubated with 10 μg/mL Gi9 for 5 minutes at 37°C before perfusion. Free rGPIa/IIa: experimental conditions were the same as for the control except that 0.1 or 1.0 μg/mL free rGPIa/IIa was present in perfusion solutions. Control mouse IgG: experimental conditions were the same as control except that rGPIa/IIa-liposomes were incubated with 10 μg/mL control mouse IgG for 5 minutes at 37°C before perfusion.
