Percentages of surface coverage of rGPIa/IIa-Ibα-liposomes on the collagen surface.
Dependence on shear rate, densities of rGPIa/IIa and rGPIbα, and VWF. Values are the mean ± SD; n = 6. (A) Percentages of surface coverage of rGPIa/IIa-Ibα-liposomes with different exofacial densities of rGPIbα, as indicated. The exofacial density of rGPIa/IIa was kept constant at 2.19 × 103 ± 0.02 × 103 molecules per particle. (B) Percentages of surface coverage of rGPIa/IIa-Ibα-liposomes with different exofacial densities of rGPIa/IIa, as indicated. The exofacial density of rGPIbα was kept constant at 1.08 × 104 ± 0.08 × 104molecules per particle. The percentages of surface coverage of rGPIbα-liposomes on the collagen surface in the presence and absence of soluble VWF (front row) were 0.07% ± 0.02% at all shear rates tested.
