NRAMP1 expression in mature granulocytes from peripheral blood (PMN leukocytes) and 3 populations of neutrophil precursors from BM.

Total RNA was extracted from (1) band 1 cells, band cells/segmented cells; (2) band 2 cells, myelocytes/metamyelocytes; and (3) band 3 cells, myeloblasts/promyelocytes and from PMN leukocytes. After separation by electrophoresis in denaturing formaldehyde gels (10 μg/lane), RNA was blotted to a hybridization membrane. The blot was probed with cDNA probes corresponding to NRAMP1 gene (A) andGAPDH gene (B) as a positive control.