Detection of the NRAMP1 c-Myc–tagged proteins in CHO and U937 cells.

(A) Western blot analysis with crude membrane extracts from untransfected CHO and U937 cells and from CHO-NRAMP1 and U937-NRAMP1 transfectants was separated by SDS-PAGE on a 10% acrylamide gel followed by transfer to PVDF membranes. Immunodetection was with affinity-purified anti-NRAMP1 (i-ii) or with anti–c-Myc (iii) antibodies. The positions and sizes (in kilodaltons) of molecular weight markers are shown. (B) Untransfected CHO cells (i,iii) and CHO-NRAMP1 (ii,iv) were processed for immunochemistry (i-ii) or immunofluorescence (iii-iv) with our affinity-purified anti-NRAMP1 antibody. Arrows indicate some NRAMP1-positive cells.