NRAMP1 association withC albicans–containing phagosomes in HL-60 cells differentiated into granulocytes.

After treatment for 5 days with DMSO, HL-60 cells were allowed to ingest heat-killed C albicans for 30 minutes at 37°C. The cells were then washed free of uningested particles, fixed, and permeabilized before indirect immunofluorescence examined by using a Nikon optical fluorescence microscope (A) or a confocal microscope (B). Detection was performed with an affinity-purified rabbit anti-NRAMP1 antibody (Ai, Bi, and Bii), nonimmune serum (Aii), or no primary antibody (Aiii) on the cells. Immunofluorescence micrographs (Ai-Aiii) and the corresponding bright field images (Aiv-Avi) are shown. Arrowhead shows an intact, particle-free, differentiated HL-60 cell. Arrows point to ingested C albicans.