RPA analysis of apoptotic mRNA after CD40 stimulation.

Daudi cells were cultured in the presence or absence (U) of either 10 μg/mL srhCD40L (L) or 10 μg/mL anti-CD40 SGN-14 (A) for 24, 48, and 72 hours before RNA extraction. RNA samples were analyzed by RPA with probes for the bcl-2 family of genes for changes in mRNA levels. Increases in bax mRNA were seen in each treatment group at each time point compared to an untreated control. Increases in other proapoptotic genes such as bcl-xL/S, bik, and bak were also seen at each time point. These data are representative of 3 experiments.