Effects of

bax antisense of Bax protein levels in lymphoma cell lines. RL cells were cultured with either srhCD40L alone, srhCD40L plus bax antisense, or media control for 48 hours. Effects on total Bax protein levels were then assessed by Western blot. Erk cytosolic protein was included as a loading control. Densitometric analysis was performed to determine the amount of Bax protein reduction from the srhCD40L-treated group relative to the Erk levels, which remained unchanged. Treatment of RL cells with bax antisense causes a reduction of Bax protein following CD40 stimulation.