Fig. 2.
Fig. 2. Generation of HA-1–specific CTLs restricted by nonself HLA-A2 molecules using DCs transduced with HA-1 cDNA. / The cells were stained with PE-conjugated HA-1A2 tetramers and with PE-Cy-5–conjugated anti-CD8 antibody at the indicated days. Appropriate gates were set on vital lymphocytes according to their typical forward- and side-scattering characteristics.

Generation of HA-1–specific CTLs restricted by nonself HLA-A2 molecules using DCs transduced with HA-1 cDNA.

The cells were stained with PE-conjugated HA-1A2 tetramers and with PE-Cy-5–conjugated anti-CD8 antibody at the indicated days. Appropriate gates were set on vital lymphocytes according to their typical forward- and side-scattering characteristics.

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