Tetramer staining and HA-1–specific cytotoxic activity of HA-1–specific CTL clones restricted by nonself HLA-A2 molecules.

(A) Staining of CTL clones with HA-1^A2 tetramers. Filled histograms represent the staining with HA-1^A2 tetramer. Open histograms represent the staining with the control HA-2^A2 tetramer. (B) Cytotoxic activity of CTL clones against HA-1 peptide–pulsed (1 μg/mL) cells and HA-1⁺EBV-LCLs in a ⁵¹Cr-release assay. The results are expressed as the mean value of the duplicate cultures at an effector-to–hot target cell ratio of 10:1.