Impact of PgP inhibition on TH9402 content in resting and activated lymphocytes.

(A) PHA-stimulated and resting lymphocytes were stained with TH9402 for 40 minutes and resuspended in medium alone or with CSA. Flow cytometric assessment of TH9402 content in CD3⁺ cells was performed 90 minutes after the end of the incubation period. Numbers in parentheses indicate the MFI of corresponding cell populations. (B) The impact of CSA exposure on the proportion of PHA-stimulated lymphocytes capable of eliminating TH9402 (MFI < 100 U) was measured over time, starting after completion of the 40-minute incubation period. The results are representative of 3 experiments.