Hepatic factor VIII and VWF mRNA levels in AOM-treated mice.

Competitive RT-PCR analysis of factor VIII (A) and VWF (B) mRNA was performed on total liver RNA from mice killed at 36 (AOM-50), 48 (AOM-30), or 72 hours (AOM-15 and saline controls), as described in “Materials and methods.” The number of cRNA copies added to the reaction is shown above the gel. DNA markers are 700, 600, 517, and 500 base pair (bp) in panel A and 517, 500, 400, and 300 bp in panel B. (C) Quantitation of competitive RT-PCR data for factor VIII (circles) and VWF (squares). Bands derived from mRNA and cRNA were stained with ethidium bromide and quantitated densitometrically. For determination of factor VIII mRNA levels, the number of mice in the control, AOM-15, AOM-30, and AOM-50 groups was 3, 3, 2, and 2, respectively. Data are expressed as mean ± sample SD.