Human Mix.1-like homeobox gene

MIXL. (A) The predicted ORF of MIXL. Nucleotides 1-285 were derived from sequencing BAC CITB-HSP-C216I11. The rest was from cDNA clones AA847809, AA911377, and AI654861. The Kozak consensus around the initiation Met are in italics. The conserved Mix-like homeodomain and carboxy-terminal domain are in bold. (B) Genomic organization of MIXL. The 1.6-kbp genomic fragment containing the intron was amplified from BAC 216I11 with the primer pair MIXL-HD, subcloned, and sequenced. The exon-intron junctions with perfect splice donor and acceptor consensus thus identified between nucleotides 448 and 449 (panel A) were confirmed by direct sequencing of BAC 216I11. Exon 1 is predicted to be more than 393 bp and exon 2 to be about 1.3 kbp. The protein domains of the predicted MIXL ORF are indicated. The amino-terminal proline-rich domain, not found in Xenopus Mix.1, is unique to MIXL and Mml.