Liver biopsy sections from affected family members stained with Perls Prussian blue.

(A) Proband, showing grade 4 iron accumulation at age 56 years. (B) Brother of the proband, showing grade 4 iron accumulation at age 73 years. (C) Son of the proband, showing grade 3 iron accumulation at age 20 years. (D) Daughter of the proband, showing grade 2 iron accumulation at age 19 years. In all patients, iron is prominent in Kupffer cells and macrophages. Iron overload is greater in the older patients, suggesting that iron accumulation is progressive with age. Original magnification × 100.