American Society of Hematology

Fig. 2.

$Fig. 2. Gene expression analysis of EBs. / (A) Gene expression in Runx1+/+ andRunx1−/− EBs. Numbers on top of the figure indicate day of EB differentiation. ES represents undifferentiated ES cells. Runx1 and β-actin expression was determined using specific oligonucleotides. Expression of other genes was evaluated using a polyA+ global amplification PCR method.46 Hybridization with a 3′ probe from the L32 ribosomal protein gene was included to control for amounts of cDNA loaded. (B) X-gal staining of Runx1+/+ andRunx1+/Z EBs. Numbers indicate day of EB differentiation. Original magnification day 0, × 400; days 2 to 4, × 200; and days 6 to 10, × 40. (C) Flow cytometry analysis of FDG stained Runx1+/+ andRunx1+/Z EBs. (D) FACS analysis ofFlk-1 and Runx1 expression in days 3, 3.5, and 4Runx1+/Z EBs. Numbers in quadrant represent the percentage of total population in each fraction.$

Gene expression analysis of EBs.

(A) Gene expression in Runx1^+/+ andRunx1^−/− EBs. Numbers on top of the figure indicate day of EB differentiation. ES represents undifferentiated ES cells. Runx1 and β-actin expression was determined using specific oligonucleotides. Expression of other genes was evaluated using a polyA⁺ global amplification PCR method.46 Hybridization with a 3′ probe from the L32 ribosomal protein gene was included to control for amounts of cDNA loaded. (B) X-gal staining of Runx1^+/+ andRunx1^+/Z EBs. Numbers indicate day of EB differentiation. Original magnification day 0, × 400; days 2 to 4, × 200; and days 6 to 10, × 40. (C) Flow cytometry analysis of FDG stained Runx1^+/+ andRunx1^+/Z EBs. (D) FACS analysis ofFlk-1 and Runx1 expression in days 3, 3.5, and 4Runx1^+/Z EBs. Numbers in quadrant represent the percentage of total population in each fraction.

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