Integrin engagement abrogates IFN-α–induced IAP-1 down-regulation.

HCs were cultured for 24 hours on plates precoated with either polyHEMA, FN, or VN with or without IFN-α (100 U/mL). (A) Whole cell lysates were separated by SDS-PAGE and Western blotted for IAP-1 and then reprobed for β-actin to control for sample loading. (B) The mean and SEM determined from 2 identical experiments using cells from 2 different HCL patients are shown. The density of the IAP-1 bands was corrected for differences in loading and related to the amount of IAP-1 present in the cells before the onset of culture (t0).