CGA, CGB, and PEA gene expression in human monocytes and neutrophils.

RT-PCR was performed by using RNA from human neutrophils (N), monocytes (M), or adrenals (A) and PEA primer pair (N1, M1, A1), CGA primer pair (N2, M2, A2), or CGB primer pair (N3, M3, A3). As a positive control for neutrophils, GAPDH was amplified (C). Negative controls were also performed by omitting RT (neutrophils C^RT−)(monocytes^RT−) from the reaction mixture. The PCR products were separated by electrophoresis on a 2% agarose gel. DNA markers were run in parallel.