American Society of Hematology

Fig. 1.

$Fig. 1. Identification of B220+ DCs. / (A) CD11c versus B220 profile of thymus low-density cell fractions. The percentages represented by B220+ CD11cint DCs and B220− CD11chi DCs and their forward scatter (FSC) versus side scatter (SSC) profiles are indicated. (B) Electron microscopic analysis of B220+ DCs. Bi: low magnification (original magnification × 2200) of MACS-purified thymic B220+ DCs. Bii: ultrastructural characteristics of B220+ DCs. B220+ DC size was 7.3 ± 0.6 μm (n = 8). (C) CD11c versus B220 profiles of spleen, lymph node, and bone marrow low-density cell fractions, showing the relative proportions of B220+ CD11cint DCs and B220− CD11chi DCs. (D) Characterization of B220+ DCs in MHC class II (MHCII) versus B220 profiles of B-cell–deficient mouse low-density cell fractions. Data are representative of 5 experiments with similar results.$

Identification of B220⁺ DCs.

(A) CD11c versus B220 profile of thymus low-density cell fractions. The percentages represented by B220⁺ CD11c^int DCs and B220⁻ CD11c^hi DCs and their forward scatter (FSC) versus side scatter (SSC) profiles are indicated. (B) Electron microscopic analysis of B220⁺ DCs. Bi: low magnification (original magnification × 2200) of MACS-purified thymic B220⁺ DCs. Bii: ultrastructural characteristics of B220⁺ DCs. B220⁺ DC size was 7.3 ± 0.6 μm (n = 8). (C) CD11c versus B220 profiles of spleen, lymph node, and bone marrow low-density cell fractions, showing the relative proportions of B220⁺ CD11c^int DCs and B220⁻ CD11c^hi DCs. (D) Characterization of B220⁺ DCs in MHC class II (MHCII) versus B220 profiles of B-cell–deficient mouse low-density cell fractions. Data are representative of 5 experiments with similar results.

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