Up-regulation of MHC and costimulatory molecules by B220⁺ DCs induced by CpG and LPS.

Histograms show the expression of the indicated markers by MACS-sorted thymic B220⁺ DCs (A) or CD8⁺ DCs (B) before culture and after 12-hour culture in control medium or in the presence of CpG or LPS. Percentage of cells with fluorescence intensity over the dashed vertical lines, corresponding to the upper limit of background staining shown in MHC class I (MHCI) histograms (white profiles), is indicated below each histogram. Mean fluorescence intensity is indicated in the upper right corner of each histogram. Data are representative of 3 experiments with similar results.