Fig. 7.
Fig. 7. T-cell tolerogenic potential of B220 DCs. / (A) OVA-TCR TC proliferation after 72-hour culture with either thymic B220+ DCs or B220− DCs (DCs) and OVA. (B) Anergy reversal assay. Proliferation of OVA-TCR TCs precultured for 72 hours with B220+ DCs (OVA-TCs[+B220+ DCs]) or B220− DCs (OVA-TCs[+DCs]) after 48-hour culture with syngeneic splenocytes and OVA in the presence of IL-2. (C) Treg cell assay. Proliferation of OVA-TCR TCs after 36-hour culture with syngeneic splenocytes and OVA in the presence of (OVA-TCs[+B220+ DCs]) or (OVA-TCs[+DCs]). See “Materials and methods” for a detailed explanation of this experimental system. Cell proliferation was assessed by [3H] thymidine (1 μCi/well) uptake in a 16-hour pulse (error bars represent the SD for triplicate cultures). Data are representative of 2 experiments with similar results.

T-cell tolerogenic potential of B220 DCs.

(A) OVA-TCR TC proliferation after 72-hour culture with either thymic B220+ DCs or B220− DCs (DCs) and OVA. (B) Anergy reversal assay. Proliferation of OVA-TCR TCs precultured for 72 hours with B220+ DCs (OVA-TCs[+B220+ DCs]) or B220− DCs (OVA-TCs[+DCs]) after 48-hour culture with syngeneic splenocytes and OVA in the presence of IL-2. (C) Treg cell assay. Proliferation of OVA-TCR TCs after 36-hour culture with syngeneic splenocytes and OVA in the presence of (OVA-TCs[+B220+ DCs]) or (OVA-TCs[+DCs]). See “Materials and methods” for a detailed explanation of this experimental system. Cell proliferation was assessed by [3H] thymidine (1 μCi/well) uptake in a 16-hour pulse (error bars represent the SD for triplicate cultures). Data are representative of 2 experiments with similar results.

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