Effect of rhC5a on production of PAI-1, t-PA, and t-PA-PAI-1 complexes in HMC-1 cells.
HMC-1 cells were incubated for 24 hours in the absence or presence of rhC5a at concentrations of 0.01, 0.1, 0.2, 0.5, 1.0, and 2.0 μM, respectively. Conditioned media of such treated cells were collected. Total PAI-1 antigen (A, ●) and active PAI-1 (A, ○), total t-PA antigen (B, ●), and t-PA activity (B, ○; 1 ng t-PA activity is equivalent to 0.5 IU t-PA) and t-PA–PAI-1 complexes (C, ●) was determined as described in “Materials and methods.” Values are given in ng/106 cells per 24 hours and represent mean values ± SD of 3 independent determinations. Experiments were performed 3 times, and a representative experiment is shown. Total PAI-1 antigen, PAI-1 activity, and t-PA–PAI-1 complexes were significantly increased by rhC5a at concentrations greater than 0. 1 μM (P < .001). t-PA activity was significantly decreased in samples obtained from cells treated with rhC5a at concentrations greater than 0.1 μM (P < .01).
