Effect of rhC5a on PAI-1 and t-PA mRNA expression in HMC-1 cells.

HMC-1 cells were incubated for 4 (lanes 1 and 2), 8 (lanes 3 and 4), and 24 hours (lanes 5 and 6) in the absence (lanes 1, 3, and 5) or presence of 1.0 mM rhC5a (lanes 2, 4, and 6). mRNA of such treated cells was prepared and PAI-1, t-PA, and GAPDH mRNA was visualized by Northern blotting, as described in “Materials and methods.” Experiments were performed twice, and a representative experiment is shown.