Fig. 6.
Fig. 6. PECAM-1−/− mice show enhanced kinetics of intracellular Ca++ responses upon IgM cross-linking. / Purified splenic B cells from wild-type and PECAM-1−/−mice were loaded with Fura-2/AM, washed, and resuspended in PBS containing 1 mM CaCl2 and 1 mM MgCl2. Calcium mobilization profiles were measured for PECAM-1+/+(---) and PECAM-1−/− (___) B cells after cross-linking BCR by stimulation with varying concentrations of anti-IgM F(ab′)2 (2.5-10 μg/mL) and calcium responses monitored in a luminescence spectrophotometer.

PECAM-1−/− mice show enhanced kinetics of intracellular Ca++ responses upon IgM cross-linking.

Purified splenic B cells from wild-type and PECAM-1−/−mice were loaded with Fura-2/AM, washed, and resuspended in PBS containing 1 mM CaCl2 and 1 mM MgCl2. Calcium mobilization profiles were measured for PECAM-1+/+(---) and PECAM-1−/− (___) B cells after cross-linking BCR by stimulation with varying concentrations of anti-IgM F(ab′)2 (2.5-10 μg/mL) and calcium responses monitored in a luminescence spectrophotometer.

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