Cytofluorimetric analysis of CD11b and F4/80 antigens in 32D/v-Abl/neo and 32D/v-Abl/β4 cells.

32D/v-Abl/neo and 32D/v-Abl/β4 cells (clone a) were stained with anti-CD11b and/or F4/80 monoclonal antibodies and analyzed by flow cytometry. (A) Samples of 1 × 10⁶ of control (neo) and β4-transduced cells were incubated with purified rat anti–mouse CD11b, and (B) with rat anti–mouse F4/80 primary antibodies. The cells were washed, incubated with FITC-conjugated secondary antibody, and analyzed by a flow cytometer after addition of 5 μL PI. (A,B) Negative controls were stained with rat anti–mouse CD4 monoclonal antibody and processed as described in “Materials and methods.”