Fig. 1.
Effect of PGE2 and cAMP modulators on STAT5 transactivation.
AS-E2 cells were transiently transfected with STAT5-luciferase reporter (STAT5-Luci) or control reporter without STAT5 binding sites (pLuci). Cells were deprived of EPO for 16 hours, pretreated with various concentrations of (A) PGE2, (B) cAMP, and IBMX for 30 minutes and subsequently cultured with or without EPO (2 U/mL) for 6 hours. Measured luciferase activities were corrected for β-galactosidase activities and expressed as percentage transactivation of EPO-stimulated control cells without pretreatment. Mean values of 3 independent experiments are presented, and SEM values are indicated by bars.
