Fig. 3.
Serine phosphorylation of STAT5 is not involved in PGE2-increased STAT5 transactivation.
AS-E2 cells that had been transfected with control vector (pcDNA3) or expressing vector for the indicated wild-type (wt) or mutant STAT5A and STAT5B proteins, were starved for 16 hours, pretreated with or without PGE2 (1 μM), and stimulated with or without EPO (2 U/mL) for 7 hours. Measured luciferase activities were corrected for β-galactosidase activities and expressed as percentage transactivation of EPO-stimulated control cells without PGE2 pretreatment. The mean values of 3 independent experiments are presented, and SEM values are indicated by bars.
