Fig. 5.
The costimulatory effect of PGE2 is mediated by PKA.
STAT5 transactivation assay of AS-E2 cells that had been cotransfected with 4 μg PKA inhibitor protein (PKI), inactive PKI (PKI mut) or control vector (pcDNA3). These cells were starved for 16 hours, pretreated with or without PGE2 (1 μM), and stimulated with or without EPO (2 U/mL) for 6 hours. Measured luciferase activities were corrected for β-galactosidase activities and expressed as percentage transactivation of unstimulated cells. The mean values of 4 independent experiments are presented, and SD values are indicated by bars.
