Fig. 6.
Involvement of CBP in STAT5 transactivation.
STAT5 transactivation assay of AS-E2 cells that had been cotransfected with 4 μg wild-type CBP, CBP Leu607Phe, adenoviral E1A, or mutant E1A proteins E1AΔCR1 and E1AΔCR2. These cells were starved for 16 hours, pretreated with or without PGE2 (1 μM), and stimulated with or without EPO (2 U/mL) for 7 hours. Measured luciferase activities were corrected for β-galactosidase activities and expressed as percentage transactivation of unstimulated cells. The mean values of 3 independent experiments are presented, and SD values are indicated by bars.
