F-Ara induces ICAM-1 expression on HMECs: protective effect of defibrotide.

Flow cytometric analysis of ICAM-1–positive cells. HMECs were either left untreated or were incubated with F-Ara (10 μg/mL or descending concentrations in B) in the presence or absence of descending concentrations of D. (A) Histogram plot of ICAM-1 expression from a representative experiment. (dotted line) Background staining (nil Control). (thin line) ICAM-1 expression of untreated control cells. (thick line) ICAM-1 expression of F-Ara–treated cells. (B) Dose-dependent induction of ICAM-1 expression by F-Ara. Summary of 3 independent experiments. Results are given as mean percentage ICAM-1–positive cells ± SD. *P = .075 of F-Ara versus untreated control cells. (C) Dose-dependent inhibition of F-Ara–induced ICAM-1 expression by defibrotide (D). Results are given as mean percentage ICAM-1–positive cells ± SD. **P = .004 of F-Ara versus F-Ara+D-treated HMECs.