Fig. 7.
F-Ara decreases the allogenicity of HMECs for NK cells: enhancement of lysis by blockade of MHC class I.
NK cells have been negatively selected (non-NK-cell–depleted) by magnetic bead separation and stimulated with irradiated HMECs in the presence of IL-2 (50 U/mL) for 4 days and subsequently subjected to a51Cr release assay as described for Figure 6. The data below the graph shows flow cytometric analysis of the effector cell population before and after stimulation with HMECs. NK cells were characterized as CD3−/CD16+CD56+. *—: percentage specific lysis of K 562 cells at an E/T ratio of 20:1.
