Analysis of cell viability and apoptosis following Bcl-2, Bcl-xL, or Mcl-1 ASO delivery.
Cells were electroporated with either Bcl-2, Bcl-xL, or Mcl-1 ASO or the mismatched related control. Then cells were resuspended in RPMI 1640 with 2% FCS. A control involving cells electroporated without ASO was performed. (A) Cell viability was determined by vital dye (eosin) exclusion, as assessed by visual inspection in a hemocytometer. (B) Apoptosis was determined by the percentage of APO 2.7–positive cells analyzed by flow cytometry. Apoptosis is expressed as the mean percentage of APO 2.7–positive cells ± SD (n = 3 at least for each condition). (C) Cell cycle analysis following electroporation of cells with Mcl-1 ASO. DNA fluorescence histograms of propidium iodide-stained myeloma cells. The cell cycle was analyzed by using the Modfit LT program (Verity Software House, Topsham, ME). The percentage of apoptotic cells represented by a sub-G1 peak is indicated.
