Characterization of Mcl-1 ASO-induced apoptosis.

(A) LP-1 cells were electroporated with either Mcl-1 ASO or the mismatched related control. Then cells were resuspended in RPMI 1640 with 2% FCS in the presence or not of z-VAD-fmk (100 μM). The percentage of APO 2.7–positive cells was determined by flow cytometry. Results are expressed as the mean ± SD of 3 experiments. (B) LP-1 cells were electroporated with either Mcl-1 ASO or the mismatched related control. Cells were recovered at the indicated time, and caspase-3 activity was determined in cell lysates by using colorimetric assay. (C) Disruption of mitochondrial membrane potential in LP-1 treated or not by MCl-1 ASO. The percentage of cells with a low ΔΨm is indicated.