Mcl-1 ASO triggered apoptosis in myeloma cells from 2 patients.

(A) Mcl-1 protein was down-regulated after treatment by Mcl-1 ASO. Cells from patients were electroporated in the presence 20 μM Mcl-1 ASO and recovered at 18 hours. Cells were then lysed, and 20 μg total protein was loaded per lane for immunoblot analysis. (B) Determination of apoptosis following Mcl-1 ASO treatment. A control involving cells electroporated without ASO was performed. After electroporation, cells were resuspended in RPMI 1640 with 10% FCS. Apoptosis induced by ASO was determined by the percentage of APO 2.7–positive cells.