APC-mediated inactivation of FVa 306Q/679Q, FVa 506Q, and FVa 506Q/679Q.

Inactivation assays were performed as described in legend to Figure 4, both in the absence (open symbols) and the presence (closed symbols) of 100 nM protein S. (A) (triangle) FVa 306Q/679Q; (diamond) FVa 306Q/506Q/679Q; (cross) control without APC. (B) (square) FVa 506Q; (diamond) FVa 506Q/679Q. The following final FVa concentrations in U/mL were used: 306Q/679Q, 0.040 ± 0.005; 306Q/506Q/679Q, 0.049 ± 0.003; WT, 0.040 ± 0.005; 506Q, 0.048 ± 0.003; and 506Q/679Q, 0.042 ± 0.0005. Plotted values represent the mean of 3 individual experiments; error bars represent SD.