MUTZ-3 DCs acquire characteristics of immature and mature DCs in the presence of cytokines.

(A) Phenotype of unstimulated MUTZ-3, MUTZ-3 iDCs, and TNFα hi, matured MUTZ-3 mDCs. Numbers refer to the percentage of positive cells stained for each CD marker. All cells were stained with PE- or FITC-labeled antigen-specific mAbs. Data are from one experiment representative of 5. (B) FACS analysis reveals up-regulation of costimulatory molecules CD80, CD86, and CD40, the adhesion molecule CD54, and HLA class II molecule HLA-DR during MUTZ-3 differentiation, unstimulated MUTZ-3 (dotted line), MUTZ-3iDC (solid line) and MUTZ-3 mDC (bold line). Isotype controls are represented by markers. Data are from one experiment representative of 5. (C) TGFβ1 induces the expression of LC-associated surface molecule langerin on MUTZ-3 cells. CD34⁺ MUTZ-3 cells were cultured in GM-CSF/TNFα followed by further culture in the presence or absence of TGFβ1. Numbers refer to the percentage of gated cells expressing both CD1a and langerin or background staining from an isotype control. Data are from one experiment representative of 3. (D) Morphology of MUTZ-3 mDCs is consistent with dendritic cell appearance (× 400, light microscopy).