Flow cytometric analysis of BM erythroid cells.

(A) Scattergram of FSC versus SSC to allow gating on cells with low FSC and low SSC properties where BM erythroid cells are included (R1). (B) Scattergram of anti-CD34 versus anti-CD71 fluorescence gated on R1, to allow gating on the CD34⁺/CD71⁺ early erythroid progenitor cells (R2). (C) Scattergram of antiglycoA versus anti-CD36 fluorescence gated on R1 to allow gating on the CD36⁺/glycoA⁺ cells (R3) and CD36⁻/glycoA⁺ cells (R4) representing the early and late erythroid precursor cells respectively. (D) Scattergram of FSC versus 7AAD fluorescence gated on R2, showing 7AAD^bright (late apoptotic), 7AAD^dim (early apoptotic), and 7AAD⁻ (live) cells. Similar scattergrams were gated on R3 and R4 regions. FSC indicates forward light scatter; SSC, right angle light scatter.