Effect of rhTNF-α on apoptotic depletion of normal BM erythroid cells.

We incubated 1 × 10⁶ BM cells from healthy subjects (n = 3) in the absence or presence of human rhTNF-α at concentrations of 10 ng/mL and 20 ng/mL for 48 hours. The percentage of apoptotic cells in the early progenitor (⋄) CD34⁺/CD71⁺, the early precursor (■) CD36⁺/glycoA⁺, and late precursor (○) CD36⁻/glycoA⁺ cell compartments were evaluated using flow cytometry and 7AAD. Each point in the diagram represents the mean (± SEM) of the 3 experiments. Comparison between treated and untreated with rhTNF-α cultures in each cell compartment was performed using the ANOVA test.