Fig. 5.
Allogenic MLR.
Allogenic MLR was performed by using purified CD4+ T cells (3 × 105 cells/well in 96-round-well plate) as responder cells. ⋄ represents Ly5.2+CD8α+Ia+ DCs from naive Ly5.2 B6 mice (wild-type CD8α+ DCs), whereas ■ represents Ly5.2+CD8α+Ia+ DCs from Ly5.2+CD8α+CD11c−Lin−precursor-reconstituted Ly5.1 B6 mice (donor-derived CD8α+ DCs). ○ represents mature DCs generated from BM-derived HPCs (BM-derived DCs). All the indicated cell subpopulations were purified or generated as described in “Materials and methods.” Results are expressed as the mean ± 1 SD of the triplicate cultures and are representative of 3 independent experiments.