Localization of donor-derived DCs in the spleen sections.

At day 14, the splenic sections were performed and double stained as described in “Materials and methods.” (A) Donor marker Ly5.2 was stained as red color, whereas CD8α marker was stained as blue color. Original magnification, × 100. (B) The same staining was performed as described in (A). Original magnifications, × 200 and × 400 (the insert picture at the right-bottom side). (C-D) Donor marker Ly5.2 was stained as red color, whereas CD11c (C) and DEC205 (D) markers were stained as blue color, respectively. Original magnifications, × 200 and × 400 (the insert pictures at the bottom right sides). The results are representative of 3 independent experiments.