The effect of the chelators on ⁵⁹Fe uptake from ⁵⁹Fe-Tf by SK-N-MC neuroepithelioma cells.

(A) The cells were incubated for 3 hours at 37°C in medium containing⁵⁹Fe-Tf (0.75 μM) and either DFO (25 μM) or the other chelators (25 μM), washed, and then incubated with pronase (1 mg/mL) for 30 minutes at 4°C to measure internalized ⁵⁹Fe. (B) The cells were incubated as described in (A) for 3, 6, 12, and 24 hours at 37°C, and internalized ⁵⁹Fe was measured using pronase. Results are expressed as the mean ± SD of 3 replicates in a typical experiment of 2 experiments performed.