The effect of the chelators on the mRNA levels ofTfR, GADD45, and β-actin (loading control) in SK-N-MC neuroepithelioma cells.

Total RNA was extracted from cells after a 20-hour incubation with medium alone (control) or medium containing DFO (150 μM) or the other chelators (25 μM). The isolated RNA was subjected to electrophoresis on a 1.2% agarose-formaldehyde gel, transferred to a hybridization membrane, and probed under high-stringency conditions (see “Materials and methods”). The result illustrated is a typical experiment from 3 experiments performed.