Dot blot presentation of forward scatter (FSC) versus side scatter (SSC) of bone marrow cells from wild-type and G-CSF–deficient mice.

The bone marrow cells were fixed with paraformaldehyde and permeabilized using acidified Tween-20 as described in “Materials and methods” and then analyzed using a FACScan. RBCs and cellular debris have been gated out. Regions were set on the white blood cell (WBC) population to define lymphocytes (R4), blasts (R3), and granulocytes (R2). Cells in regions R2 and R3 were sorted, and cytospin preparations of these cells were stained with May-Grünwald-Giemsa. The morphology of these cells is shown in the bottom panel. Panels A and B represent cells in region R3, and panels C and D represent cells in region R2 from wild-type and G-CSF–deficient mice, respectively.