Fig. 3.
Fig. 3. Initial BrdU uptake by various subpopulations of bone marrow cells and their distribution in bone marrow. / Wild-type and G-CSF–deficient mice were injected with BrdU (intraperitoneally), and after 2 hours they were killed. The bone marrow cells were fixed and permeabilized and then labeled with anti–BrdU-FITC antibody followed by Gr-1-PE staining. The total height of each column represents the percentage of total cells. The unshaded portions represent labeled cells (cells in DNA synthesis). BrdU uptake by blast cells from G-CSF–deficient mice was significantly higher than that from wild-type mice (P < .05). HSSC represents cells with high side scatter in FSC versus SSC FACS profile. Data are represented as mean ± SEM (n = 3) and are a representative plot of 1 of the 3 independent experiments.

Initial BrdU uptake by various subpopulations of bone marrow cells and their distribution in bone marrow.

Wild-type and G-CSF–deficient mice were injected with BrdU (intraperitoneally), and after 2 hours they were killed. The bone marrow cells were fixed and permeabilized and then labeled with anti–BrdU-FITC antibody followed by Gr-1-PE staining. The total height of each column represents the percentage of total cells. The unshaded portions represent labeled cells (cells in DNA synthesis). BrdU uptake by blast cells from G-CSF–deficient mice was significantly higher than that from wild-type mice (P < .05). HSSC represents cells with high side scatter in FSC versus SSC FACS profile. Data are represented as mean ± SEM (n = 3) and are a representative plot of 1 of the 3 independent experiments.

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