Cell-cycle analysis of bone marrow cells from wild-type and G-CSF–deficient mice.

Mice of each genotype were injected with BrdU intraperitoneally. After 2 hours they were killed, and the bone marrow cells were harvested. The bone marrow cells were then fixed and permeabilized and labeled with anti–BrdU-FITC antibody. The cells were further stained with PI and then analyzed in a FACScan machine using Cell Quest software. The relative DNA content of a cell as measured by PI uptake is represented on the x-axis, and the cells in S-phase as determined by BrdU uptake are represented on the y-axis. The left-hand panel shows the plot for total WBCs, and the right-hand panel represents the plot for blast cells.